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1.1mg / ml ( by BCA assay )

PBS cell lysate , freeze / thawed and clarified by centrifugation 

PBS + 0.1% Triton x-100

0.1% Triton x-100 . Tested by attempted passage in permissive cells showing no detectableCPE for 5 days

Avoid repeated freeze / thawing Vortex before use . If needed , can be sonicated

Store frozen at less than-20°C , preferably-80°C