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Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM172; H3K27ac, Histone H3 (K27 acetylation)

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Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM172; H3K27ac, Histone H3 (K27 acetylation)

品牌：RevMAb

貨號(hào)：31-1056-00

規(guī)格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM172]
Product Description	Rabbit monoclonal to Acetylated Histone H3 Lysine 27 (K27ac), H3K27ac, Histone H3 (K27 acetylation)
Catalog No.	31-1056-00
Clone Name	RM172
Product Data Sheet	Anti-Acetyl-Histone H3 Lys27 Rabbit Monoclonal Antibody Data Sheet RM172
Specificity	RM172 reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27).
Application	Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex Chromatin IP (ChIP)
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
.
Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL - 2 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
References for Acetyl-Histone H3 (Lys27) antibody [RM172]	Li Y. et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. 113(50):14402-14407. ChIP; Human. Read more (PubMed: 27911794)
Product Images
	RM172 specifically reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
	Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM172 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys27) Rabbit mAb RM172 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).