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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Androsterone (ADT) ELISA Kit
品牌:Biomatik
貨號:EKU02347
規(guī)格:96T
貨期:
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Androsterone (ADT) ELISA Kit

商品詳情 參考文獻(xiàn) 相關(guān)資料

Product Description

Species Reactivity Multispecies (General)
Format 96 well plate
Detection Range 352-90000 pg/mL
Sensitivity 144 pg/mL
Sample Type Serum, plasma and other biological fluids
Assay Time 2 hours
Gene Name Androsterone
Gene Aliases 17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone
Application ELISA
Assay Type Competitive
Detection Method Colorimetric
Shipping Condition Blue ice
Storage Instructions -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date 8 months
Specificity This assay has high sensitivity and excellent specificity for detection of Androsterone (ADT). No significant cross-reactivity or interference between Androsterone (ADT) and analogues was observed.
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androsterone (ADT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androsterone (ADT) and unlabeled Androsterone (ADT) (Standards or samples) with the pre-coated antibody specific to Androsterone (ADT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androsterone (ADT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androsterone (ADT) in the sample.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androsterone (ADT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions For research use only. Not for diagnostic procedures.
Quality Systems The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

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